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This assay is used for evaluating patients with suspected Hypogonadism, predicting ovulation, evaluating Infertility and diagnosing Pituitary disorders. This assay is also an adjunct in the evaluation of menstrual irregularities. In both males & females Primary hypogonadism results in elevated levels of basal LH & FSH. LH is decreased in Primary ovarian hyperfunction in females & Primary hypergonadism in males.