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Serum iron, TIBC & Percent saturation are widely used for the diagnosis of Iron deficiency. This assay is useful for screening Chronic iron overload diseases particulary Hemochromatosis. Percent saturation is usually normal or increased in Iron deficiency, Pregnancy & intake of oral contraceptives. Low TIBC is seen in Chronic inflammatory conditions, Hemochromatosis & Malignancies. Serum ferritin is a more sensitive & reliable indicator of Iron deficiency.